Salvianolic Acid B Suppress Maturation of Dendritic Cells

Sal B effects on human monocyte-derived dendritic cell maturation blocked by PPARγ siRNA

FRIDAY, Dec. 2 (HealthDay News) -- Salvianolic acid B (Sal B) -- a herb-derived, water-soluble antioxidant known to help prevent atherosclerosis -- effectively suppresses the maturation of human monocyte-derived dendritic cells (h-monDCs), according to a study published in the December issue of the British Journal of Pharmacology.

Aijun Sun, M.D., Ph.D., from Fudan University in Shanghai, China, and colleagues investigated whether the anti-atherosclerotic effect of Sal B is mediated by suppressing h-monDC maturation. Purified human monocytes were incubated with granulocyte macrophage-colony stimulating factor and interleukin (IL)-4 to derive h-monDCs. The h-monDCs were stimulated by oxidized low-density lipoprotein (ox-LDL) in the presence or absence of peroxisome proliferator-activated receptor-γ (PPARγ) small interfering RNA (siRNA), after being pre-incubated with or without Sal B. Fluorescence-activated cell sorting was used to analyze the expression of h-monDC membrane molecules (CD40, CD86, CD1a, HLA-DR), an enzyme linked immunosorbent assay measured the cytokines, and Western blotting was used to determine the Toll-like receptor (TLR) 4-associated signalling pathway.

The investigators found that ox-LDL had multiple effects, including promotion of h-monDC maturation; stimulation of CD40, CD86, CD1a, and HLA-DR expression; production of IL-12, IL-10, and tumor necrosis factor-alpha; and up-regulation of TLR4 signalling. Sal B inhibited these effects. PPARγ activation, promotion of PPARγ nuclear translocation, attenuation of ox-LDL-induced up-regulation of TLR4 and myeloid differentiation primary-response protein 88, and inhibition of the downstream p38-mitogen activated protein kinase signalling cascade were also mediated by Sal B. These effects of Sal B were blocked by knocking down PPARγ with the corresponding siRNA.

"The possible anti-atherosclerotic effects of Sal B might be mediated by suppressing maturation of h-monDC," the authors write.

Full Text (subscription or payment may be required)

Copyright © 2011 HealthDay. All rights reserved.

Powered by

jQuery UI Accordion - Default functionality

For life-long learning and continuing professional development, come to Lippincott's NursingCenter.

Nursing Jobs Plus
Featured Jobs
Recommended CE Articles

Debunking Three Rape Myths
Journal of Forensic Nursing, October/December 2014
Expires: 12/31/2016 CE:2.5 $24.95

Drug updates and approvals: 2014 in review
The Nurse Practitioner, 13December 2014
Expires: 12/31/2016 CE:3 $27.95

Can Food Processing Enhance Cancer Protection?
Nutrition Today, September/October 2014
Expires: 10/31/2016 CE:2 $21.95

More CE Articles

Subscribe to Recommended CE

Recommended Nursing Articles Evidence Based Practice Skin Care Network NursingCenter Quick Links What’s Trending Events