Interaction of RNA Binding Proteins ID'd in Glioblastoma

HuR regulation of Msi1 occurs at the post-transcriptional level in glioblastoma cells

WEDNESDAY, Jan. 18 (HealthDay News) -- The RNA-binding protein (RBP) HuR interacts with and regulates Musashi1 (Msi1) post-transcriptionally in glioblastoma cells, according to an experimental study published in the January issue of Molecular Cancer Research.

Dat T. Vo, from the University of Texas Health Science Center at San Antonio, and colleagues investigated the interaction between HuR and Msi1 in glioblastoma cells. Quantitative real time polymerase chain reactions were used to measure HuR and Msi1 in clinical glioblastoma samples.

The researchers found that HuR interacted with the 3'-untranslated region of Msi1, positively regulating Msi1 expression. HuR increased mRNA stability and promoted translation of MSI1. Expression of HuR and Msi1 were positively associated in glioblastoma samples. Silencing of HuR resulted in inhibition of cell proliferation, increases in apoptosis, and changes in the cell-cycle profile, which were partially rescued by ectopic expression of Msi1.

"The HuR:Msi1 relationship discussed in this article creates a positive cascade network motif where the cellular logic for gliomagenesis requires HuR to essentially "activate" Msi1 gene expression," the authors write. "The interaction between these two RBPs creates a two-component composite gene regulatory network."

Abstract
Full Text (subscription or payment may be required)

Copyright © 2012 HealthDay. All rights reserved.

Powered by

jQuery UI Accordion - Default functionality

For life-long learning and continuing professional development, come to Lippincott's NursingCenter.

Nursing Jobs Plus
Featured Jobs
Recommended CE Articles

Blunt Chest Trauma
Journal of Trauma Nursing, November/December 2014
Expires: 12/31/2016 CE:2 $21.95


The School Age Child with Congenital Heart Disease
MCN, The American Journal of Maternal/Child Nursing, January/February 2015
Expires: 2/28/2017 CE:2.5 $24.95


Understanding multiple myeloma
Nursing Made Incredibly Easy!, January/February 2015
Expires: 2/28/2017 CE:2 $21.95


More CE Articles

Subscribe to Recommended CE

Recommended Nursing Articles

Comprehensive Care: Looking Beyond the Presenting Problem
Journal of Christian Nursing, January/March 2015
Free access will expire on March 2, 2015.


Pain and Alzheimer dementia: A largely unrecognized problem
Nursing Made Incredibly Easy!, January/February 2015
Free access will expire on February 16, 2015.


Glycemic control in hospitalized patients
Nursing2015 Critical Care, January 2015
Free access will expire on February 16, 2015.


More Recommended Articles

Subscribe to Recommended Articles

Evidence Based Practice Skin Care Network NursingCenter Quick Links What’s Trending Events