Genetic Profile of Circulating Tumor Cells Is Heterogeneous

Rare cells isolated from blood have distinct profile from single cells from cancer cell lines

FRIDAY, May 11 (HealthDay News) -- Gene expression of circulating tumor cells (CTCs), rare cells in the blood of patients with solid tumors, is heterogeneous and is distinct from the profiles of single cells from cancer cell lines commonly used to study cancer, according to a study published online May 7 in PLoS One.

Ashley A. Powell, Ph.D., from the Stanford University School of Medicine in California, and colleagues used an immunomagnetic enrichment device that isolates live tumor cells from unfractionated blood, the MagSweeper, to purify CTCs. They examined the expression of 87 cancer-associated and reference genes in single CTCs from patients with primary and metastatic breast cancer.

The researchers found that gene expression was heterogeneous in the single CTCs. Based on 31 genes that were highly expressed, CTCs were divided into two major subgroups. In contrast, the single cells from seven breast cancer cell lines were tightly clustered, with expression profiles distinct from the CTCs.

"For the first time, we directly measured high dimensional gene expression in individual CTCs without the common practice of pooling such cells," Powell and colleagues conclude. "Our findings demonstrate that profiling CTCs on a cell-by-cell basis is possible and may facilitate the application of 'liquid biopsies' to better model drug discovery."

Several authors disclosed financial ties to the MagSweeper technology (licensed to Illumina, where one author is an employee). Another author is a co-founder of the Fluidigm Corporation, manufacturer of the chips used in the study.

Full Text

Copyright © 2012 HealthDay. All rights reserved.

Powered by

jQuery UI Accordion - Default functionality

For life-long learning and continuing professional development, come to Lippincott's NursingCenter.

Nursing Jobs Plus
Featured Jobs
Recommended CE Articles

Blunt Chest Trauma
Journal of Trauma Nursing, November/December 2014
Expires: 12/31/2016 CE:2 $21.95

The School Age Child with Congenital Heart Disease
MCN, The American Journal of Maternal/Child Nursing, January/February 2015
Expires: 2/28/2017 CE:2.5 $24.95

Understanding multiple myeloma
Nursing Made Incredibly Easy!, January/February 2015
Expires: 2/28/2017 CE:2 $21.95

More CE Articles

Subscribe to Recommended CE

Recommended Nursing Articles

Comprehensive Care: Looking Beyond the Presenting Problem
Journal of Christian Nursing, January/March 2015
Free access will expire on March 2, 2015.

Pain and Alzheimer dementia: A largely unrecognized problem
Nursing Made Incredibly Easy!, January/February 2015
Free access will expire on February 16, 2015.

Glycemic control in hospitalized patients
Nursing2015 Critical Care, January 2015
Free access will expire on February 16, 2015.

More Recommended Articles

Subscribe to Recommended Articles

Evidence Based Practice Skin Care Network NursingCenter Quick Links What’s Trending Events