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Head and neck cancer is the sixth most common cancer in the world. The vast majority of cases are head and neck squamous cell carcinoma (HNSCC), a type of cancer that arises in the outer layer of the skin and mucous membranes of the mouth, nose, and throat. Cigarette smoking is a major risk factor for developing the disease and reduces treatment effectiveness.

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Now Thomas Jefferson University researchers studying the effects of cigarette smoke on tumor progression show that cigarette smoke reprograms the cells surrounding the cancer cells and helps drive HNSCC aggressiveness. The results were published online in the journal Molecular Cancer Research (2019; doi: 10.1158/1541-7786.MCR-18-1191).


"Cigarette smoke changes the metabolism of cells in head and neck squamous cell carcinomas, making the tumors more efficient as an ecosystem to promote cancer growth," noted Ubaldo Martinez-Outschoorn, MD, Associate Professor in the Department of Medical Oncology and a researcher at the Sidney Kimmel Cancer Center- Jefferson Health, who led the new study.


In previous research, Martinez-Outschoorn and colleagues including Joseph Curry, MD, Associate Professor in the Department of Otolaryngology-Head and Neck Surgery found that interactions between fibroblasts and cancer cells promote tumor growth.


"We found previously that tumors in human and animal models of head and neck squamous cell carcinoma thrive when these distinct groups of cells support each other," said Martinez-Outschoorn. Cancer cells make use of metabolic products generated by the surrounding fibroblasts in order to obtain energy and fuel their growth. "That's where tumors are most aggressive."


Marina Domingo-Vidal, a graduate student in Martinez-Outschoorn's lab, studied the metabolic effects of tobacco in head and neck squamous cell carcinoma. "Knowing that cigarette smoke is the strongest risk factor for this cancer type, we wanted to better understand how it changes the metabolism of the different cells in the tumor," Domingo-Vidal stated.


Study Details

In the current study, the researchers exposed fibroblasts to cigarette smoke. They saw that fibroblasts increased a particular type of metabolism called glycolysis, which produces metabolites that are used by the nearby cancer cells to help fuel their growth. In addition, these cancer cells acquired certain features of malignancy such as increased mobility and resistance to cell death. The enhanced support of the tobacco-exposed fibroblasts caused larger tumors in a mouse model of the disease.


In a key finding, the researcher also found a protein on tobacco-exposed fibroblasts that appeared to drive these metabolic changes. "The protein, called monocarboxylate transporter 4 (MCT4), is a major mechanism by which cigarette smoke exerts cancer aggressiveness, and we've shown how to manipulate and hopefully reverse it," Domingo-Vidal explained.


"We've also seen that smoke-exposed fibroblasts interact with other cells in the tumor stroma, such as the cells of the immune system," she said. "A healthy immune system is responsible for recognizing and attacking malignant cells, so it will now be interesting to understand how these altered fibroblasts might influence the efficacy of current immunotherapies."


The discovery has set the foundation for a clinical trial where Curry hopes to shut down the negative metabolic state induced by cigarette smoke. "Our finding is part of a growing interest in understanding the metabolic relationship between different cells in the tumor microenvironment, and how we can target them to improve patient outcomes," Curry stated.


The clinical trial will use a two-pronged approach to combat the cancer. The trial will combine an FDA-approved drug for diabetes called metformin that will target the cancer cells' altered metabolism with the FDA-approved immunotherapy durvalumab, a checkpoint or PD-L1 inhibitor that takes the brakes off the immune system (NCT03618654).


"We think metformin and durvalumab might have a synergistic effect on the cancer, where metformin slows the bad players, the cancer cells, and durvalumab grows the strength of the good players, the immune cells," Curry concluded.


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